Palmitoylethanolamide ameliorates hippocampal damage and behavioral dysfunction after perinatal asphyxia in the immature rat brain



Versión PDF

Autor:
Herrera, María I.
Udovin, Lucas Daniel
Toro-Urrego, Nicolás
Kusnier, Carlos
Luaces, Juan P.
Capani, Francisco

Título: Palmitoylethanolamide ameliorates hippocampal damage and behavioral dysfunction after perinatal asphyxia in the immature rat brain


Frontiers Media

Palabras clave:
COMPORTAMIENTO
ASFIXIA PRENATAL
NEUROPSICOLOGIA
TRASTORNOS DEL NEURODESARROLLO

Frontiers in Neuroscience, 12:145, 2018

ISSN 1662-453X

Disponibilidad: Acceso abierto

Cita: Herrera, M. I., Udovin, L. D., Toro-Urrego, N., Kusnier, C. F., Luaces, J. P., y Capani, F. (2018). Palmitoylethanolamide ameliorates hippocampal damage and behavioral dysfunction after perinatal asphyxia in the immature rat brain [en línea] Frontiers in Neuroscience, 12:145. doi: 10.3389/fnins.2018.00145 Disponible en: https://www.frontiersin.org/articles/10.3389/fnins.2018.00145/full Registro disponible en: http://bibliotecadigital.uca.edu.ar/greenstone/cgi-bin/library.cgi?a=d&c=investigacion&d=palmitoylethanolamide-ameliorates-hippocampal-damage [Fecha de consulta: ….]

Descripción: Abstract: Perinatal asphyxia (PA) is an obstetric complication associated with an impaired gas exchange. This health problem continues to be a determinant of neonatal mortality and neurodevelopmental disorders. Palmitoylethanolamide (PEA) has exerted neuroprotection in several models of brain injury and neurodegeneration. We aimed at evaluating the potential neuroprotective role of PEA in an experimental model, which induces PA in the immature rat brain. PA was induced by placing Sprague Dawley newborn rats in a water bath at 37°C for 19 min. Once their physiological conditions improved, they were given to surrogate mothers that had delivered normally within the last 24 h. The control group was represented by non-fostered vaginally delivered pups, mimicking the clinical situation. Treatment with PEA (10 mg/kg) was administered within the first hour of life. Modifications in the hippocampus were analyzed with conventional electron microscopy, immunohistochemistry (for NeuN, pNF-H/M, MAP-2, and GFAP) and western blot (for pNF H/M, MAP-2, and GFAP). Behavior was also studied throughout Open Field (OF) Test, Passive Avoidance (PA) Task and Elevated Plus Maze (EPM) Test. After 1 month of the PA insult, we observed neuronal nucleus degeneration in CA1 using electron microscopy. Immunohistochemistry revealed a significant increase in pNF-H/M and decrease in MAP-2 in CA1 reactive area. These changes were also observed when analyzing the level of expression of these markers by western blot. Vertical exploration impairments and anxiety-related behaviors were encountered in the OF and EPM tests. PEA treatment attenuated PA-induced hippocampal damage and its corresponding behavioral alterations. These results contribute to the elucidation of PEA neuroprotective role after PA and the future establishment of therapeutic strategies for the developing brain.

Fil: Herrera, María I. Universidad Católica Argentina. Facultad de Psicología y Psicopedagogía. Centro de Investigaciones en Psicología y Psicopedagogía; Argentina

Fil: Udovin, Lucas Daniel. Universidad de Buenos Aires. Instituto de Investigaciones Cardiológicas; Argentina

Fil: Udovin, Lucas Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina

Fil: Toro-Urrego, Nicolás. Universidad de Buenos Aires. Instituto de Investigaciones Cardiológicas; Argentina

Fil: Toro-Urrego, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina

Fil: Kusnier, Carlos. Universidad de Buenos Aires. Instituto de Investigaciones Cardiológicas; Argentina

Fil: Kusnier, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina

Fil: Luaces, Juan P. Universidad de Buenos Aires. Instituto de Investigaciones Cardiológicas; Argentina

Fil: Luaces, Juan P. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina

Fil: Capani, Francisco. Universidad de Buenos Aires. Instituto de Investigaciones Cardiológicas; Argentina

Fil: Capani, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina

Fil: Capani, Francisco. Universidad Católica Argentina. Facultad de Medicina; Argentina

Fil: Capani, Francisco. Universidad Autónoma de Chile; Chile
Ver texto completo: Ver el documento (formato PDF)
Estadísticas a partir del año 2017

Descargas Mensuales:

Distribución geográfica de los visitantes:

Pontificia Universidad Católica Argentina   -  Av. Alicia Moreau de Justo 1300, C1107AAZ, Buenos Aires, Argentina     +54 11 4349 0200